Name: ANDRÉIA LOPES DE MORAIS

Publication date: 26/07/2019
Advisor:

Namesort descending Role
MOISÉS ZUCOLOTO Advisor *

Examining board:

Namesort descending Role
MOISÉS ZUCOLOTO Advisor *

Summary: MORAIS, Andreia Lopes; M.Sc.; Federal University of Espirito Santo; July 2019;
Growth, production and diversity of the 'Tahiti' acid file combined with different
rootstocks; Advisor: Dr. Moises Zucoloto.

Established definitively in the south of the state of Florida in the year 1875, popularly
known as lemon, the acidic 'Tahiti' file is likely to originate in Tahiti, an island of French
Polynesia, this plant belongs to the family Rutaceae, Citrus genus and species Citrus
Latifolia Tanaka. In the present day there is a great challenge in the production of fruit
trees, which mainly consists of the diversification of rootstock for citrus. The
accentuated use of the same rootstock can promote the occurrence of adversities,
such as susceptibility to diseases. In addition, crown varieties represent the productive
potential of the crop and present differential behaviors when combined with rootstocks.
In this way, two chapters were developed analyzing the production, growth and
diversity of 'Tahiti' acid file cups combined with different rootstocks. In the first chapter,
the objective of this study was to evaluate the vegetative growth and the production of
'Tahiti' (Bello Fruit, Elhão, Iconha, Itarana, Santa Rosa, Bearss Lime, BRS Passos,
Persian 58, CNPMF 01, CNPMF 02, CNPMF 2001, and CNPMF 5059) combined with

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two rootstocks, Citrumelo Swingle and Citrandarin Riverside. Evaluations were carried
out during two years for the following characteristics: vegetative development;
production and productive efficiency; internal and external quality of the fruits. The
crowps Bello Fruit, Iconha, BRS Passos, Itarana and Persian 58, CNPMF 5059, and
Éledio were superior for most characteristics evaluated. The Citrumelo Swingle
rootstock was the one that induced better results for vegetative characteristics such as
rootstock and graft diameter, line diameter, interline and plant height. The Citrandarin
Riverside rootstock induced higher yields for the canopies studied. In the second
chapter, the objective was to determine and compare the genetic divergence in twelve
varieties of 'Tahiti' acid file on two rootstocks, by means of morphological markers and
molecular markers. The 'Tahiti' acidic genotypes evaluated for genetic divergence
were the same as those cited above in each of the rootstocks, Citrandarin Riverside
and Citrumelo Swingle, using six quantitative descriptors (height, stem diameter, stem
diameter five centimeters down and above the line of grafting, diameter in the line and
planting interline), for the morphoagronomic characteristics. For the determination of
genetic divergence via molecular, sheets of each of the twelve canopies studied were
collected and 12 ISSR primers were used. By the morphoagronomic analysis it was
verified the existence of the variability among the genotypes. In which, for the plants
grafted on both the Citrumelo Swingle and Citrandarin Riverside rootstock, four groups
were formed for each respective rootstock. For the molecular analyzes, the accesses
with the lowest genetic distance observed by the dissimilarity values were among the
individuals Iconha and Bearss Lime, and the greatest distance between the genotypes
Éledio and CNPMF 02.

Keywords: Citrus Latifolia Tanaka, genetic divergence, asexual propagation.

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