Name: DRIELLY STEPHÂNIA GOUVÊA
Publication date: 28/06/2016
Advisor:
Name |
Role |
|---|---|
| RODRIGO SOBREIRA ALEXANDRE | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| EDILSON ROMAIS SCHMILDT | Co advisor * |
| JOÃO PAULO BESTETE DE OLIVEIRA | External Examiner * |
| RODRIGO SOBREIRA ALEXANDRE | Advisor * |
Summary: The Somatic embryogenesis is the process of development of embryos from somatic
cells. The objective of this work to analyze the growth regulators efficiency in different
stages of indirect somatic embryogenesis from in vitro plants of hermaphrodite
papaya hybrid UENF / Caliman 01. Leaf discs of these shoots were inoculated in
induction (MI): MS basal medium (Murashige, Skoog, 1962) with a total concentration
of salts and supplemented with auxins 2,4-dichlorophenoxyacetic acid (2,4-D) (6; 9;
12; 15:18 μM) and 4-chlorophenoxyacetic acid (4-CPA) (19; 22; 25; 28 and 31 μM).
The treatments were arranged in a completely randomized design (CRD) with five
repetitions, five explants per repetition. The data were submitted to analysis of
variance by F test and means were compared by Skott-Knott test at the 5% level of
significance. During 90 days of induction, visualized training some somatic embryos
formed in friable callus. The concentrations of 2,4-D auxin were inhibitory, occurring
only the formation of light-brown callus colored, which were lower than 20% results in
the formation of somatic embryos, WHEREas supplementation with 4-CPA resulted in
96% embryogenic callus for the concentration of 25 μM. The embryogenic calli were
transferred to the maturation medium (MM): MS without growth regulators; ABA -
abiscísico acid (0,5 μM); ABA (0,5 μM) + CA - activated charcoal (15 g L-1); ABA (0,5
xi
μM) + CA (30 g L-1); ABA (0,5 μM) + PEG - polyethylene glycol (60 g L-1) for 30 days.
Significant differences were observed among the tested maturation media, a positive
reaction to the amount of developed embryos and the effect of each treatment. The
maturation medium consisting of 0,5 μM ABA CA + 30 g L-1 was the most efficient in
the development of cotyledonary somatic embryos. These mature cotyledon embryos
were inoculated in germination (MG): MS without growth regulators; GA3 (0,5 mg L-1);
GA3 (1,0 mg L-1) and GA3 (1,5 mg L-1).
